分子集合系計算科学セミナーを 10/10 に開催
題目：Macromolecular Crowding Beyond Hard-Core Repulsions
講師：Daniel Harries（The Hebrew University of Jerusalem）
日時：10月10日（木）17：00－
場所：ハイブリッド開催（大阪大学豊中キャンパス基礎工学研究棟講義室A304，Zoom）
要旨：In contrast to the highly crowded solution inside cells, proteins are almost always studied in dilute aqueous buffer. I will review some of the experimental evidence that crowding affects the equilibrium thermodynamics of protein stability and protein association and discuss the theories employed to explain these observations. Theories based on hard-core interactions predict only crowding-induced entropic stabilization. However, experiment-based efforts conducted under physiologically relevant conditions show that crowding can destabilize proteins and their complexes. Furthermore, quantification of the temperature dependence of crowding effects produced by both large and small cosolutes, including osmolytes, sugars, synthetic polymers, and proteins, reveals enthalpic effects that stabilize or destabilize proteins. The emergent role of chemical interactions between and among the protein, cosolutes, and water augments the known effect of hard-core interactions and explains the thermodynamic footprint of crowding on protein stability and association. Applications to dilute and concentrated solutions will be discussed.
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世話人：笠原健人（大阪大），石井良樹（北里大），肥喜里志門（立命館大），吉田悠一郎（大阪大）
分子集合系計算科学セミナーHP: https://sites.google.com/view/bunsisyugo/
